A method for isolating and culturing placental cells from failed early equine pregnancies

Rose, B V and Cabrera-Sharp, V and Firth, M J and Barrelet, F E and Bate, S and Cameron, I J and Crabtree, J R and Crowhurst, J and McGladdery, A J and Neal, H and Pynn, J and Pynn, O D and Smith, C and Wise, Z and Verheyen, K L P and Wathes, D C and De Mestre, A M (2016) A method for isolating and culturing placental cells from failed early equine pregnancies. PLACENTA, 38. pp. 107-111.

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Abstract

Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.