Silver, M J and Corbin, K D and Hellenthal, G and Da Costa, K A and Dominguez-Salas, P and Moore, S E and Owen, J and Prentice, A M and Hennig, B J and Zeishel, S H (2015) Evidence for Negative Selection at SNPs Increasing Dietary Choline Requirement in a Gambian Cohort. FASEB JOURNAL, 29 (8). pp. 3426-35.
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Abstract
Choline is an essential nutrient, and the amount needed in the diet is modulated by several factors. Given geographical differences in dietary choline intake and disparate frequencies of single-nucleotide polymorphisms (SNPs) in choline metabolism genes between ethnic groups, we tested the hypothesis that 3 SNPs that increase dependence on dietary choline would be under negative selection pressure in settings where choline intake is low: choline dehydrogenase (CHDH) rs12676, methylenetetrahydrofolate reductase 1 (MTHFD1) rs2236225, and phosphatidylethanolamine-N-methyltransferase (PEMT) rs12325817. Evidence of negative selection was assessed in 2 populations: one in The Gambia, West Africa, where there is historic evidence of a choline-poor diet, and the other in the United States, with a comparatively choline-rich diet. We used 2 independent methods, and confirmation of our hypothesis was sought via a comparison with SNP data from the Maasai, an East African population with a genetic background similar to that of Gambians but with a traditional diet that is higher in choline. Our results show that frequencies of SNPs known to increase dependence on dietary choline are significantly reduced in the low-choline setting of The Gambia. Our findings suggest that adequate intake levels of choline may have to be reevaluated in different ethnic groups and highlight a possible approach for identifying novel functional SNPs under the influence of dietary selective pressure.
| Item Type: | Article |
|---|---|
| RVC Publication Type: | Research (full) paper |
| DOI: | https://doi.org/10.1096/fj.15-271056 |
| Departments: | Pathology and Pathogen Biology |
| Research Programmes: | Livestock Production and Health |
| Depositing User: | RVC Auto-import |
| Last Modified: | 21 Feb 2020 02:07 |
| URI: | http://researchonline.rvc.ac.uk/id/eprint/9590 |
| Date Deposited: | 23 January 2019 |