Yamakawa, Y and Pennelegion, C and Willcocks, S and Stalker, A and MacHugh, N and Burt, D and Coffey, T J and Werling, D (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN. Journal of Leukocyte Biology, 83 (6). pp. 1396-1403.
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Abstract
Dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) C-type lectin is almost exclusively expressed at the cell surface of DC. In addition to its normal function facilitating contact of DC with T cells, DC-SIGN has been shown to bind a variety of pathogens, including <i>Mycobacterium bovis</i>, and HIV-1 envelope protein gp120. In this study, we identified the bovine ortholog of the human DC-SIGN gene within the bovine genome, which exists as a single copy. PCR amplified a product, showing a 100% match with the predicted sequences as well as a sequence predicted to be similar to that of SIGNR7. Furthermore, a protein with the same molecular weight as human DC-SIGN was detected by Western blot in cell lysate derived from bovine DC. To characterize this molecule functionally, the uptake of FITC-labeled OVA and FITC-labeled gp120 (FITC-gp120) by bovine and human DC was assessed. FITC-gp120 was shown to bind to bovine DC in a time- and temperature-dependent manner. Binding was blocked by a polyclonal anti-DC-SIGN antibody but not by a control antibody. Furthermore, blocking of this molecule also reduced the binding of <i>M. bovis</i> bacillus Calmette-Guerin expressing GFP. Confocal microscopy showed that DC-SIGN was expressed on the surface of bovine DC. Subsequent pulse-chase studies revealed that FITC-gp120 was internalized by bovine monocyte-derived DC as early as 10 min. Thus, there is evidence of a DC-SIGN-like molecule expressed specifically by bovine DC. This molecule may play an important role in the infection of bovine (DC) cells with <i>M. bovis</i>.
| Item Type: | Article |
|---|---|
| RVC Publication Type: | Research (full) paper |
| WoS ID: | 000258019800012 |
| DOI: | https://doi.org/10.1189/jlb.0807523 |
| Departments: | Pathology and Pathogen Biology |
| Research Programmes: | Livestock Production and Health > Infection and Immunity |
| Depositing User: | RVC Auto-import |
| Last Modified: | 21 Feb 2020 12:56 |
| URI: | http://researchonline.rvc.ac.uk/id/eprint/1761 |