Berhane, Y and Bailey, S R and Putignano, C and Elliott, J (2008) Characterization of agonist-induced endothelium-dependent vasodilatory responses in the vascular bed of the equine digit. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 31 (1). pp. 1-8.
Full text not available from this repository.Abstract
The role of endothelium-derived relaxing factors was studied in the regulation of vascular responses in the Krebs perfused equine isolated digit. Perfusion pressure was recorded in response to bolus doses of 5-hydroxytryptamine (6 nmol) alone or co-administered with carbachol (CCh; 0.2 mu mol), bradykinin (BK; 0.2 nmol), substance P (SP; 0.2 nmol) or sodium nitroprusside (SNP; 0.2 mu mol). N-omega-Nitro-L-Arginine methyl ester hydrochloride (L-NAME; 300 mu M) caused partial but significant inhibition of CCh-induced vasodilatory response, whereas BK and SP-induced responses were resistant to L-NAME. High potassium (K+, 30 mM) and the cytochrome P-450 (CYP) epoxygenase inhibitor, clotrimazole (10 mu M) plus L-NAME (100 mu M), completely abolished the CCh, BK and SP-induced vasodilatory responses, whereas the response to SNP was unaffected. In contrast, the L-NAME-resistant proportion of CCh, BK and SP-induced vasodilatory response was not inhibited by the highly selective CYP2C9 inhibitor, sulphaphenazole (10 mu M). The cyclo-oxygenase inhibitor, ibuprofen (10 mu M) did not affect the CCh, BK and SP-induced responses. These data demonstrate that CCh, BK and SP-induced relaxation in the equine digit involve a combination of the NO and endothelium-derived hyperpolarizing factor (EDHF) pathways. These results do not support the evidence for the involvement of CYP-derived epoxyeicosatrienoic acids and the exact nature of EDHF in the equine digit remains to be established.
| Item Type: | Article |
|---|---|
| RVC Publication Type: | Research (full) paper |
| WoS ID: | 000251862300001 |
| DOI: | https://doi.org/10.1111/j.1365-2885.2007.00912.x |
| Departments: | Comparative Biomedical Sciences |
| Research Programmes: | Comparative Physiology & Medicine > Cardiovascular and Inflammation Biology and Metabolism |
| Depositing User: | RVC Auto-import |
| Last Modified: | 21 Nov 2020 15:21 |
| URI: | https://researchonline.rvc.ac.uk/id/eprint/1340 |
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