Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices

Adams, C F and Delaney, A M and Carwardine, D R and Tickle, J and Granger, N and Chari, D M (2018) Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices. Macromolecular Bioscience, 19 (2).

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Abstract

A recent clinical trial proved that autologous olfactory mucosal cell (OMC) transplantation improved locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs responded to the treatment, likely due to the transplantation procedures involving injections of cell suspensions that are associated with cell death, uneven cell distribution and cell washout. Encapsulating cells in protective hydrogel matrices offers a tissue engineering solution to safely achieve 3-D growth of viable transplant cells for implantation into injury sites, to improve regenerative outcomes. We show for the first time that canine OMCs can be propagated with high viability in 3-D collagen matrices. Further, we describe a method to incorporate canine OMCs pre-labelled with clinical grade iron-oxide nanoparticles into the constructs. Intra-construct labelled cells could be visualised using magnetic resonance imaging, offering substantial promise for in vivo tracking of cOMCs delivered in protective matrices.

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