The Effect of Escherichia coli Lipopolysaccharide and Tumour Necrosis Factor Alpha on Ovarian Function

Williams, E J and Sibley, K and Miller, A N and Lane, E A and Fishwick, J C and Nash, D M and Herath, S and England, G C W and Dobson, H and Sheldon, I M (2008) The Effect of Escherichia coli Lipopolysaccharide and Tumour Necrosis Factor Alpha on Ovarian Function. American Journal of Reproductive Immunology, 60 (5). pp. 462-473.

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Abstract

Problem Pelvic inflammatory disease and metritis are important causes of infertility in humans and domestic animals. Uterine infection with Escherichia coli in cattle is associated with reduced ovarian follicle growth and decreased estradiol secretion. We hypothesized that this effect could be mediated by the bacterial lipopolysaccharide (LPS) or cytokines such as tumour necrosis factor alpha (TNF alpha). Method of study In vitro, bovine ovarian theca and granulosa cells were treated with LPS or TNF alpha and steroid secretion measured. In vivo, the effect of LPS or TNF alpha intrauterine infusion was determined by ovarian ultrasonography and measurement of hormones in cattle. Results Lipopolysaccharide reduced granulosa cell estradiol secretion, whilst TNF alpha decreased theca and granulosa cell androstenedione and estradiol production, respectively. In vivo, fewer animals ovulated following intrauterine infusion with LPS or TNF alpha. Conclusion Lipopolysaccharide and TNF alpha suppress ovarian cell function, supporting the concept that pelvic inflammatory disease and metritis are detrimental for bovine ovarian health.