Plasmid selection in Escherichia coli using an endogenous essential gene marker

Goh, S and Good, L (2008) Plasmid selection in Escherichia coli using an endogenous essential gene marker. Bmc Biotechnology, 8 (61).

[img] Text
1464.pdf
Restricted to Repository staff only

Download (1MB)

Abstract

Background: Antibiotic resistance genes are widely used for selection of recombinant bacteria, but their use risks contributing to the spread of antibiotic resistance. In particular, the practice is inappropriate for some intrinsically resistant bacteria and in vaccine production, and costly for industrial scale production. Non-antibiotic systems are available, but require mutant host strains, defined media or expensive reagents. An unexplored concept is over-expression of a host essential gene to enable selection in the presence of a chemical inhibitor of the gene product. To test this idea in E. coli, we used the growth essential target gene fabI as the plasmid-borne marker and the biocide triclosan as the selective agent.