Isolation and generation of osteoblasts

Perpetuo, I P and Bourne, L E and Orriss, I R (2019) Isolation and generation of osteoblasts. In: Bone Research Protocols. Humana Press, New York. ISBN 978-1-4939-8997-3

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Abstract

This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.