Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor beta 1

Lawson, J S and Syme, H M and Wheeler-Jones, C P D and Elliott, J (2018) Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor beta 1. BMC Veterinary Research, 14. p. 76.

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Abstract

Chronic kidney disease (CKD) is common in geriatric cats, and the most prevalent pathology is chronic tubulointerstitial inflammation and fibrosis. The cell type predominantly responsible for the production of extra-cellular matrix in renal fibrosis is the myofibroblast, and fibroblast to myofibroblast differentiation is probably a crucial event. The cytokine TGF-β1 is reportedly the most important regulator of myofibroblastic differentiation in other species. The aim of this study was to isolate and characterise renal fibroblasts from cadaverous kidney tissue of cats with and without CKD, and to investigate the transcriptional response to TGF-β1.