Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12

Bossé, J T and Li, Y and Angen, Ø and Weinert, L A and Chaudhuri, R R and Holden, M T and Williamson, S M and Maskell, D J and Tucker, A W and Wren, B W and Rycroft, A N and Langford, P R (2014) Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12. JOURNAL OF CLINICAL MICROBIOLOGY, 52. pp. 2380-2385.

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Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.