RVC Research Online

Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12

Bossé, J T and Li, Y and Angen, Ø and Weinert, L A and Chaudhuri, R R and Holden, M T and Williamson, S M and Maskell, D J and Tucker, A W and Wren, B W and Rycroft, A N and Langford, P R (2014) Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12. JOURNAL OF CLINICAL MICROBIOLOGY, 52. pp. 2380-2385.

[img]
Preview
Text
8557.pdf - Published Version
Available under License Creative Commons Attribution.

Download (658kB) | Preview

Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

Item Type: Article
RVC Publication Type: Research (full) paper
WoS ID: 000339279700015
DOI: https://doi.org/10.1128/JCM.00685-14
Departments: Pathobiology and Population Sciences
Pathology and Pathogen Biology
Research Programmes: Livestock Production and Health
Depositing User: RVC Auto-import
Date Deposited: 12 Nov 2014 16:23
Last Modified: 30 Nov 2018 11:27
URI: http://researchonline.rvc.ac.uk/id/eprint/8557